Structural and Functional Studies of Aspergillus clavatus N5-Carboxyaminoimidazole Ribonucleotide Synthetase,
نویسندگان
چکیده
منابع مشابه
Structural and functional studies of Aspergillus clavatus N(5)-carboxyaminoimidazole ribonucleotide synthetase .
N(5)-Carboxyaminoimidazole ribonucleotide synthetase (N(5)-CAIR synthetase), a key enzyme in microbial de novo purine biosynthesis, catalyzes the conversion of aminoimidazole ribonucleotide (AIR) to N(5)-CAIR. To date, this enzyme has been observed only in microorganisms, and thus, it represents an ideal target for antimicrobial drug development. Here we report the cloning, crystallization, and...
متن کاملStructural analysis of the active site geometry of N5-carboxyaminoimidazole ribonucleotide synthetase from Escherichia coli.
N(5)-Carboxyaminoimidazole ribonucleotide synthetase (N(5)-CAIR synthetase) converts 5-aminoimidazole ribonucleotide (AIR), MgATP, and bicarbonate into N(5)-CAIR, MgADP, and P(i). The enzyme is required for de novo purine biosynthesis in microbes yet is not found in humans suggesting that it represents an ideal and unexplored target for antimicrobial drug design. Here we report the X-ray struct...
متن کاملNovel inhibitors of the bacterial de novo purine biosynthesis enzymes, n5-carboxyaminoimidazole ribonucleotide synthetase and mutase
NOVEL INHIBITORS OF THE BACTERIAL DE NOVO PURINEBIOSYNTHESIS ENZYMES, N-CARBOXYAMINOIMIDAZOLERIBONUCLEOTIDE SYNTHETASE AND MUTASEbyMARIA V FAWAZAugust 2012 Advisor: Dr. Steven M. FirestineMajor: Pharmaceutical SciencesDegree: Master of ScienceAntibiotic resistance has seen a significant increase during the past decade. Theincreasing frequency of the drug-...
متن کاملEnzymology And Medicinal Chemistry Of N5-Carboxyaminoimidazole Ribonucleotide Synthetase : A Novel Antibacterial Target
ENZYMOLOGY AND MEDICINAL CHEMISTRY OF N-CARBOXYAMINOIMIDAZOLE RIBONUCLEOTIDE SYNTHETASE:A NOVEL ANTIBACTERIAL TARGETbyHANUMANTHARAO PARITALAAUGUST 2010Advisor: Dr. Steven M. FirestineMajor: Pharmaceutical SciencesDegree: Doctor of PhilosophyN-Carboxyaminoimidazole ribonucleotide synthetase (N-CAIRsynthetase), a key enzyme in microbial de novo purine b...
متن کاملThree-dimensional structure of N5-carboxyaminoimidazole ribonucleotide synthetase: a member of the ATP grasp protein superfamily.
Escherichia coli PurK, a dimeric N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) synthetase, catalyzes the conversion of 5-aminoimidazole ribonucleotide (AIR), ATP, and bicarbonate to N5-CAIR, ADP, and Pi. Crystallization of both a sulfate-liganded and the MgADP-liganded E. coli PurK has resulted in structures at 2.1 and 2.5 A resolution, respectively. PurK belongs to the ATP grasp superfamil...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemistry
سال: 2010
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi901599u